Tech Update: New Fluorescent Nanovascules for Intracellular Biomarker Discovery TOU

New Fluorescent Nanovascules for Intracellular Biomarker Discovery

Schematic presentation of DNA-grafted QS nanovasics. QSs loaded with organic fluorophore (i.e., donor) are prepared, and fluorescent 5′-end cholesterol-triethylene glycol (TEG) modified DNA probe (i.e., F-miRNA probe) Is added to the colloidal system. Assembled in membrane. The inclusion of a fluorescent amphiphilic DNA probe inside the nanovascular membrane results in a freight between the embedded donor (D) and the acceptor on the probe (F). In the presence of a specific target miRNA, the hybridization event produces a single-to-double strand structural change in the DNA probe, resulting in a decrease in FRET efficiency due to an increase in the average distance between the FRET donor and the receiver. Credit: DOI: 10.1002 / adfm.202103511

The new work of the Nanomol Group, affiliated with the CIBER-BBN network, in collaboration with a team from the University of Rome Tor Vergata, introduces new nanovasics capable of crossing biological barriers such as cell membranes, while retaining their sensory capacity, making them sensitive. Attractive probes for intracellular detection of biomarkers.

“The development of probes capable of sensing the biological environment and signaling the presence of a specific target molecule is a challenge with compatibility in a variety of biomedical applications, including diagnostic tools from drug delivery,” says Mariana Cober, ICMAB researcher and co-author. Study, with Nora Ventosa, of ICMAB, and Alessandro Porchetta, University of Rome Tor Vergata.

This work, which has been published in Advanced functional contentRepresents the design of working fluorescent nanowicles with biomimetic DNA capable of translating their bonding with target molecules into optical output, through first-resonance energy transfer (FRET) and changes in fluorescent emissions.

These quatsomes (QS) nanowicles are an emerging class of highly stable small unilemelar vesicles of -150–100 nm diameter, formed by self-assembly of ionic surfactants and sterols in aqueous media. Their high stability, even in body fluids, uniformity and particle-to-particle uniformity make them an attractive soft material for sensing application. “QS nanowicles are loaded with fluorescent probes based on amphiphilic nucleic acids to produce programmable FRET-enabled nanowicles that act as highly sensitive signal transducers,” the researchers explain.

CIBER-BBN researchers have participated in characterizing the photophysical properties of these nanovasics and demonstrated highly selective detection of clinically related microRNAs with sensitivity in the nanomolar range. This production of nanowicles by ICMAB-CSIC’s Biomaterials Processing and Nanostructuring Unit and their physical-chemical characteristic is due to the services of ICTS NANBIOSIS.

According to the authors, the proposed strategy for the detection of various biomarkers could be easily adapted: “We hope to acquire a wide range of nucleic acids and other medically related molecules for detection of molecules in body fluids or directly in cells, thanks to Quatsomes’ ability for intracellular delivery.” . ”


Extracellular vesicles can be individual drug delivery vehicles

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                                                                                            <strong>More info:</strong>
                                            Mariana Rossetti et al., Engineering DNA-grafted quatsomes as stable nucleic acid-responsive fluorescent nanoveocycles, <i>Advanced functional content</i> (2021).  <a data-doi="1" href="http://dx.doi.org/10.1002/adfm.202103511" target="_blank" rel="noopener">DOI: 10.1002 / adfm.202103511</a>



                                            Provided by the Institute of Materials Science, Barcelona

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