Stem cell assembly by injection for cartilage regeneration TOU

Stem cell assembly by injection for cartilage regeneration

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Stem cell assembly by injection for cartilage regeneration

2 Project chart of NTs. c) In the FESEM film, most BMSCs form interactions with other cells and structures such as 1D fiber, which were similar to the structures of natural tissue. D) By redesigning the antioxidant microenvironment, improving cell viability and chondrogenesis of transplanted cells, cartilage regeneration can finally be achieved. Credit: Science China Press “width =” 800 “height =” 530″/>

a) Project chart of the 3D TGFβ-BMSC-IHI nanoscopy. b) Schematic diagram of gelatin coated and TGF-β3-loaded MnO2 NDs. c) In the FESEM film, most BMSCs form interactions with other cells and structures such as 1D fiber, which were similar to the structures of natural tissue. D) By redesigning the antioxidant microenvironment, improving cell viability and chondrogenesis of transplanted cells, cartilage regeneration can finally be achieved. Credit: Science China Press

A study led by Professor Qiu Zhang (Northwestern Polytechnic University), Professor Ki-Pum Lee (Rutgers University), and Professor Liang Kang (School of Stomatology, The Fourth Military Medical University), has established the injectable hybridIH (IH). Used for nanoscaffold-tempered stem cell assembly and regeneration of critical scale cartilage defects.

Cartilage lesions are often destructive and most of them have not healed due to the inherently low regenerative capacity of cartilage tissue. The rise of 3D stem cell culture systems has led to advances in biology, disease modeling and regenerative medicine. For example, stem cells, once successfully transplanted, may initially secrete trophic factors that reduce inflammation at the site of cartilage injury and then differentiate into cartilage cells (e.g. chondrocytes) for functional restoration.

Nevertheless, there are important barriers that need to be addressed before realizing the therapeutic potential of stem cell therapy. Limited control over the chondrogenic differentiation of stem cells in vivo often resulted in compromised regenerative effects. Furthermore, stem cells often undergo apoptosis after injection, due to antioxidant pressure and the spread of inflammation in the microenvironment of the wound site.

To meet these challenges, the researchers demonstrated the development of a 3D IHI nanoscaffold-templated stem cell assembly system for advanced 3D stem cell culture and implantation. The 3D-IHI nanoscopholder rapidly integrates stem cells through 3D cell-cell and cell-matrix interactions into the implanted tissue structure, delivering chondrogenic proteins to deeply and uniformly assembled 3D cultural systems, and controlling the effects of nanotopogenesis.

Once fitted to the rabbit cartilage injury model in vivo, the 3D-IHI nanoscopold effectively modifies the microscopic environment after the cartilage injury by combining the aforementioned regenerative references and simultaneously reacting with manganese dioxide-based compound oxygen. In this way, the acceleration of cartilage defects through rapid tissue remodeling and functional recovery is felt both in the short term and in the long term. Based on the excellent versatility and therapeutic effects of 3D-IHI nanoscopfol based cartilage regeneration, it can provide promising avenues for advancing various tissue engineering applications.

Published in research National Scientific Research.

  • Stem cell assembly by injection for cartilage regeneration

    A) A schematic diagram showing a 3D-IHI nanoscopy can enhance the chromogenic differentiation of BMSC by synergy between N-cadre and FAK-mediated pathways. b) Strong connections between MnO2 NTs and functional groups, which are common in ECM proteins, effectively supported cell proliferation demonstrated by the SEM film. C) Bisconic acid rating indicates enhanced absorption from MnO to gelatin2 Nanotube compared to control groups. D) MnO2 The nanotube-template assembly method significantly improved cell-matrix relationships demonstrated by over-regulated expression patterns of the FAK gene. C) Representative immunostaining images showing enhanced chondrogenesis of BMSC in the BMSC-IHI nanoscopy group compared with control groups. Scale: 50 m. fh) The expression of chondrogenic genes including SOX9 (f), Aggrecan (g) and Col-II (h) was characterized by qRT-PCR measurement. Credit: Science China Press

  • Stem cell assembly by injection for cartilage regeneration

    a) Schematic diagram illustrating the chronological repair surgical procedure and timeline. Decomposition of MnO2 NTs and the regenerative process can be monitored via MRI. b) To identify our transplanted cells, BMSCs were genetically labeled with green fluorescent protein (GFP). Scale: 100 m. c) The ROS study revealed a dramatically reduced red luminous signals MnO2 NTs in IHI nanoscopes can effectively remove ROS in the defective area. Stimulated cell proliferation was confirmed by high expression of the proliferative marker of G67 immunostaining. Scale: 50 m. d) By counting the number of remaining GFP + cells in the TGFβ-BMSC-IHI nanoscophold (c) a significant number of cells can be retained after transplantation compared to other cell transplant groups. e) Histogram of luminous intensity of ROS study shows effective consumption of ROS in MnO2 NTs with groups. f) Measurement of Ki67 + cells in defects. The scales in (c) and (f) are based on the luminous intensities in (c). Credit: Science China Press

  • Stem cell assembly by injection for cartilage regeneration

    a) Schematic diagram illustrating the long-term (3 months) cartilage regeneration process. b) In vivo cartilage regeneration was characterized by H&E, Safranin O staining, Col-II immunochemistry staining and macroscopic views. Zoom bar size: 2mm, zoom bar size: 200 m. ch) Cartilage thickness (by H&E staining) (c), cellular components (by Safranin O staining) (d), ECM components (by Col II immunostaining) (e). The results of the International Cartilage Repair Association (ICRS) macroscopic (f) and histological scores (g) indicate significantly improved defect repair properties in the TGFβ-BMSC-IHI nanoscopold group. Decreased Osteoarthritis Research Society International (OARSI) scores revealed that TGFβ-BMSC-IHI nanoscopold prevents the worsening of arthritis (h). Credit: Science China Press


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More info:
Zhenqiang Wang et al., Injectable hybrid mineral nanoscopold, as a rapid stem cell assembly template for cartilage repair National Scientific Research (2022) DOI: 10.1093 / nsr / nwac037

Presented by Science China Press

Quote: Cartilage Regeneration (April 15, 2022) Injectable Stem Cell Assembly Retrieved on April 15, 2022 from https://phys.org/news/2022-04-stem-cell-cartilage-regeneration.html

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