Nanoparticles can carry out highly sensitive and durable rapid Covid-19 testing TOU

Nanoparticles can carry out highly sensitive and durable rapid Covid-19 testing

 TOU

Nanoparticles can carry out highly sensitive and durable rapid Covid-19 testing

ACS sensors Adaptation from 2022, DOI: 10.1021 / acssensors.2c00100 “width =” 800 “height =” 530 “/>

A 3D-printed device detects temperature changes when SARS-CoV-2 binds to molecularly printed polymer nanoparticles (ruler shows cm). Credit: Adapted from ACS sensors 2022, DOI: 10.1021 / Acssensors.2c00100

Rapid antigen tests can quickly and conveniently tell a person that they are positive for Covit-19. However, since antibody-based tests are not very sensitive, they may fail to detect early infections with low viral loads. Now, researchers say ACS sensors Instead of antibodies to detect SARS-CoV-2, rapid tests have been developed that use molecularly printed polymer nanoparticles. The new test is more sensitive and operates under more extreme conditions than antibody-based tests.

The gold standard test for Covit-19 diagnosis is the reverse transcription-polymerase chain reaction (RT-PCR). Although this test is highly sensitive and specific, it usually takes 1-2 days to get results, which is expensive and requires specialized laboratory equipment and trained personnel. In contrast, rapid antigen tests are faster (15-30 minutes) and people can take them home without any training. However, they are not sensitive, which sometimes results in false negatives. Furthermore, tests use antibodies against SARS-CoV-2 for detection, which can withstand a wide range of temperatures and pH. Marlos Peters and Jack McClement at the University of Newcastle, Francesco Conforta and colleagues at MIP Discovery wanted to perform the low-cost, rapid, robust, and highly sensitive Covid-19 test, which uses molecularly embedded polymer nanoparticles instead of antibodies.

The researchers created the nanomiphis by making molecular seals or molds on the nanoparticles against a small piece of SARS-CoV-2 spike protein or peptide. These nano-sized binding cavities had the appropriate size and shape to identify and bind the printed peptide and, therefore, the entire protein. They attached nanoparticles very strongly bonded to the peptide to the printed electrodes. After showing that nanoMIPs can bind SARS-CoV-2, they developed a 3D-printed prototype device that detects virus binding by measuring changes in temperature.

When the team added samples from seven patient nasopharyngeal swabs to the device, the fluid flowed over the electrode, and the researchers found changes in the temperature of samples previously tested positive for COVID-19 by RT-PCR. The test only took 15 minutes, and preliminary results indicated that SARS-CoV-2 levels could be detected 6,000 times lower than a commercial rapid antigen test. Unlike antibodies, nanoMIPs can withstand warmer temperatures — providing longer life for testing in hot climates — and acidic pH — which can be useful for monitoring SARS-CoV-2 in wastewater and saliva samples. However, to prove that the test has a lower false negative rate than the current rapid antigen tests, the researchers say it still needs to be tested in several patient samples.


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                                                                                            <strong>More info:</strong>
                                            Molecularly embedded polymer nanoparticles enable rapid, reliable and robust point-of-core thermal detection of SARS-CoV ‑ 2, <i>ACS sensors</i> (2022) <a data-doi="1" href="https://dx.doi.org/10.1021/acssensors.2c00100" target="_blank" rel="noopener">DOI: 10.1021 / acssensors.2c00100</a>


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                                             <strong>Quote</strong>: Nanoparticles can activate high-sensitivity and durable rapid Covid-19 test (2022, April 13)





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